Faculty of Agro-Industry PSU

Academic Staff

FACULTY OF AGRO-INDUSTRY

Dr. Aem Nuylert

Affiliation :

Contact:

Research record:

  • ResearchGate
  • Web of Science ResearcherID:

Education :

  • 2009 B.Sc. (Agro-Industry), Prince of Songkla University, Thailand
  • 2012 M.Sc. (Biotechnology), Prince of Songkla University, Thailand
  • 2020 Ph.D. (Biotechnology Engineering), Toyama Prefectural University, Japan

Course Director :

Field of interest :

  • Discovery the interesting enzymes for food industry and fine chemical industry.
  • Production, purification, characterization of plants and microbial enzymes and its application
  • Production the recombinant enzymes using molecular genetics techniques.
  • Enzyme engineering using molecular dynamics approach

Current researches :

  • Value-added fermented split gill mushroom using co-starter culture GABA-producing and flavor-enhancing bacteria
  • Screening for fungicide substance to control the growth of Ganoderma spp. causing basal stem rot in oil palm
  • Comparison of soil microbial diversity with basal stem rot of oil palm trees and normal soil in southern Thailand
  • Biogenic-amines content and selection of biogenic amines-degrading strains from traditional fermented meat and fishery products

Awards :

Publications :

1. Nuylert, A. and Hongpattarakere, T. 2013. Improvement of cell-bound lipase from Rhodotorula mucilaginosa P11I89 using as methanol-tolerant whole-cell biocatalysts for production of palm-oil biodiesel. Ann. Microbiol. 63(3): 929–939.
2. Hongpattarakere, T., Buntin, N. and Nuylert, A. 2016. Histamine development and bacterial diversity in microbially-challenged tonggol (Thunnus tonggol) under temperature abuse during canning manufacture. J. Food Sci. Technol. 53(1): 245–256.
3. Motojima, F., Nuylert, A., and Asano, Y. 2016. Crystal structure of hydroxynitrile lyase from passion fruit, Passiflora edulis. New Biotechnol. 33, (Supplement) S96.
4. Nuylert, A., Ishida, Y., and Asano, Y. 2016. Contribution of N-glycosylation site to the enzyme stability of hydroxynitrile lyase from Passiflora edulis in the differential expression system. New Biotechnol. 33, (Supplement) S96.
5. Nuylert A, Ishida Y, and Asano Y. 2017. Effect of glycosylation on the biocatalytic properties of hydroxynitrile lyase from the passion fruit, Passiflora edulis: A comparison of natural and recombinant enzymes. ChemBioChem 18(3):257-265.
6. Motojima, F., Nuylert, A. and Asano, Y. 2017. The crystal structure and catalytic mechanism of hydroxynitrile lyase from passion fruit, Passiflora edulis, FEBS J., 285(2), 313-324. DOI: 10.1111/febs.14339.
7. Yamaguchi, T., Nuylert, A., Ina, A., Tanabe, T. and Asano, Y. 2018. Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile, Sci. Rep. 8, 3051. DOI:10.1038/s41598-018-20190-x.
8. Nuylert, A., Kuwahara, Y., Hongpattarakere, T. and Asano, Y. 2018. Identification of saturated and unsaturated 1-methoxyalkanes from the Thai millipede Orthomorpha communis as potential “Raincoat Compounds”. Sci. Rep. 8: 11730. DOI:10.1038/s41598-018-30156-8.
9. Zhai, Z. Nuylert, A., Isobe, K., and Asano, Y. 2019. Effects of codon optimization and glycosylation on the high-level production of hydroxynitrile lyase from Chamberlinius hualienensis in Pichia pastoris. J. Ind. Microbiol. Biotechnol., 46 (7). 887-898.
10. Nuylert, A., Motojima, F., Khanongnuch, C., Hongpattarakere, T. and Asano, Y. 2020. Stabilization of hydroxynitrile lyases from two variants of passion fruit, Passiflora edulis Sims and Passiflora edulis Forma flavicarpa, by C-terminal truncation. ChemBioChem. 21(1-2), 181-189. doi:10.1002/cbic.201900468.
11. A. Nuylert, M. Nakabayashi, T. Yamaguchi, and Y. Asano. 2020. Discovery and structural analysis to improve the enantioselectivity of hydroxynitrile lyase from Parafontaria laminata millipedes for (R)-2-chloromandelonitrile synthesis, ACS Omega, 5 (43), 27896-27908. doi: 10.1021/acsomega.0c03070.
12. Motojima, F., Izumi, A., Nuylert, A., Zhai, Z., Dadashipour, M., Shichida, S., Yamaguchi, T., Nakano, S. and Asano, Y. 2021. R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis—A new entry to the carrier protein family Lipocalines. FEBS J. 288(5), 1679-1695.